Associate Scientist - Molecular Biology and Assay Development

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Associate Scientist - Molecular Biology and Assay Development

Broadmead Medical Services Inc

icon Fremont, CA, US, 94538

iconFull Time

icon7 November 2024

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Company Description

Jobs for Humanity is collaborating with USD 437 to build an inclusive and just employment ecosystem. We support individuals coming from all walks of life.

Company Name: USD 437

Job Description

Company Description

Eurofins Scientific is an international life sciences company, providing a unique range of analytical testing services to clients across multiple industries, to make life and our environment safer, healthier and more sustainable. From the food you eat, to the water you drink, to the medicines you rely on, Eurofins laboratories work with the biggest companies in the world to ensure the products they supply are safe, their ingredients are authentic and labelling is accurate.

The Eurofins network of companies is the global leader in food, environment, pharmaceutical and cosmetic product testing and in agroscience Contract Research Organisation services. It is one of the market leaders in certain testing and laboratory services for genomics, discovery pharmacology, forensics, advanced material sciences and in the support of clinical studies, as well as having an emerging global presence in Contract Development and Manufacturing Organisations. It also has a rapidly developing presence in highly specialised and molecular clinical diagnostic testing and in-vitro diagnostic products.

In over 30 years, Eurofins has grown from one laboratory in Nantes, France to 58,000 staff across a decentralised and entrepreneurial network of 900 laboratories in over 54 countries. Eurofins companies offer a portfolio of over 200,000 analytical methods to evaluate the safety, identity, composition, authenticity, origin, traceability and purity of biological substances and products.

In 2021, Eurofins generated total revenues of EUR 6.72 billion, and has been among the best performing stocks in Europe over the past 20 years.

Job Description

Become your most extraordinary self!  Our team is intensely proud to be a major provider of services and products to the drug discovery research industry.  Join Eurofins DiscoverX Products, LLC part of the Eurofins Discovery ™ group of companies serving global clients in the Pharma and Biotech industries.  Work alongside industry experts and collaborate with team members to directly and positively impact human health worldwide!

We have a philosophy to support work life balance, career progression opportunities and offer many benefits such as formal mentoring programs, tuition reimbursement, extensive career training programs, competitive health benefits, paid time off and 10 annual paid holidays*.  

Essential Duties and Responsibilities:

  • Actively maintain the Eurofins DiscoverX plasmid collection including fulfilling requests for plasmids and generating additional quantities of plasmids by performing DNA preps (in-house or via out-sourcing).
  • Design mammalian expression constructs: identify the correct gene sequence (according to the workplan); optimize the DNA sequence (codon usage) and confirm in-frame fusion with tags (if applicable).
  • Use bioinformatics tools (including SnapGene, NCBI databases, etc) for executing and confirming cloning projects.
  • Generate expression plasmids required for all mammalian cell-based assay development activities at Eurofins DiscoverX.  
  • Routinely submit samples to, and evaluate data returned by, our external DNA sequencing partner
  • Perform PCR on genomic DNA from cell lines to confirm stable integration of genes and quantitative PCR to confirm gene expression.
  • Perform RNA extraction and quantify gene expression by qRT-PCR.
  • Generate Biosafety Documentation.
  • Perform routine cell culture, as well as stable pool and clone development of engineered cells.
  • Perform characterization of engineered cell lines using molecular, biochemical, and cell biology techniques, including flow cytometry, qPCR, Western blotting, immunofluorescence or other functional readouts.
  • Perform cell-based assays using established Eurofins DiscoverX assays to test activity of client molecules and report results to manager in appropriate format(s).
  • Accurately prepares and presents experimental data at regular group meetings.
  • Works diligently to meet product development goals set by management team.
  • Provides support functions to the R&D team such as ordering and maintenance of reagents/supplies/equipment, maintaining accurate notes and records, and effective communication.
  • Performs all duties in compliance with all company procedures and regulations.
  • Ability to multitask and communicate effectively to ensure orderly completion and transfer of plasmids to downstream assay development projects.
  • Maintains good documentation of cloning and laboratory work using various internal databases and electronic lab notebook.
Qualifications

To perform this job successfully, the individual must be able to perform each essential duty satisfactorily. The requirements below are representative of the knowledge, skills or ability required.

  • Minimum of 1-3 years as a molecular biology contributor is required.
  • Knowledge and demonstrated use of basic methods for molecular biology (restriction enzymes, subcloning, agarose gels, analysis of DNA sequencing, minipreps, PCR)
  • Use bioinformatics tools (including SnapGene and NCBI databases etc) for executing and confirming cloning projects.
  • Experience with mammalian cell culture (preferably with both adherent and suspension cells)
  • Ability to perform experiments and analyze resulting data, with minimal guidance from supervisor.  Shows some ability to troubleshoot existing protocols.
  • Ability to work simultaneously on multiple projects in a fast-paced, high-volume work environment is important.
  • B.S. or M.S. in molecular biology, cell biology, biochemistry, immunology or related discipline.
  • Experience planning and troubleshooting subcloning projects is preferred.
  • Experience in quantitative PCR (qPCR) and reverse transcriptase-PCR (qRT-PCR) is desirable.
  • Work with various modes of gene delivery (virus, lipid & electroporation) including retroviral or lentiviral vector systems is desirable.
  • Industry experience is preferred.
  • Skills in any or all of the following are desirable: Western blotting, immunofluorescence microscopy and Flow cytometry.
  • Excellent verbal and written communication skills are essential.
  • The qualified candidate will be a highly motivated individual with excellent organizational and communication skills, with proven ability to multitask and work under demanding timelines.
  • Must be comfortable and willing to work as both an individual contributor as well as an interactive team member.
Additional Information

Candidates within a commutable distance to Fremont, CA are encouraged to apply. The shift for this role will be: Monday-Friday, 8:00AM - 5:00PM.

Salary Range: $75,000-$90,000/yr. 

  • Excellent full time benefits including comprehensive medical coverage, dental, and vision options
  • Life and disability insurance
  • 401(k) with company match
  • Paid vacation and holidays